Dear CCp4 users, May anyone help me in interpreting the self-rotation function from molrep. Data can be indexed,scaled equally well in P21 and P222. This protein has two domain linked by a long flexible linker and get cleaved during crystallization. After crystallizing it in many condition, i believed that i found new condition where it may be full length. May anyone please suggest me by looking at the self-rotation function, how many molecule exist in ASU. Matthews coefficient suggest that there would be 2 copy of full length protein or four of truncated protein in P21 space group. For your interpretation, please find attached images of rotation function around K=180 Molrep self rotation peaks are P21 space group theta phi chi P(i)/P(0)| +------------------------------------------+ | 1 0.00 0.00 0.00 1.00 | | 2 90.00 -0.00 180.00 0.79 | | 3 35.78 -0.00 180.00 0.17 | | 4 90.00 -170.05 180.00 0.17 | | 5 58.71 180.00 180.00 0.14 | | 6 108.34 180.00 180.00 0.14 | | 7 117.39 180.00 180.00 0.13 | | 8 90.00 90.00 108.54 0.13 | | 9 90.00 -90.00 108.54 0.13 | | 10 144.50 -0.00 180.00 0.13 | | 11 37.57 25.59 179.98 0.12 | | 12 148.10 0.00 180.00 0.12 | | 13 121.73 6.36 179.62 0.11 | | 14 63.31 -42.27 180.00 0.11 | | 15 90.00 90.00 162.29 0.11 | | 16 90.00 -90.00 162.29 0.11 | | 17 71.12 136.88 180.00 0.10 | | 18 99.17 -180.00 180.00 0.10 | | 19 144.80 -161.87 180.00 0.10 | | 20 90.00 -138.24 180.00 0.10 | | 21 82.27 -76.45 180.00 0.10 | | 22 90.00 90.00 115.94 0.10 | | 23 90.00 -90.00 115.94 0.10 | | 24 159.16 27.07 180.00 0.10 | | 25 95.08 -35.92 179.84 0.10 | | 26 113.86 -139.44 179.54 0.10 | | 27 90.23 -0.00 89.32 0.10 | | 28 20.18 -156.63 179.98 0.10 | | 29 116.90 -40.40 179.50 0.10 | | 30 63.12 139.60 179.50 0.10 P222 space group theta phi chi P(i)/P(0)| +------------------------------------------+ | 1 0.00 0.00 0.00 1.00 | | 2 90.00 -21.41 180.00 0.13 | | 3 125.32 0.00 180.00 0.13 | | 4 151.18 -90.00 180.00 0.12 | | 5 90.00 -180.00 90.00 0.12 | | 6 141.32 26.82 180.00 0.12 | | 7 90.00 -42.59 180.00 0.11 | | 8 127.22 -25.21 180.00 0.11 | | 9 37.04 -14.48 180.00 0.11 | | 10 59.17 -129.14 180.00 0.10 | | 11 56.66 -174.09 180.00 0.09 | | 12 40.85 144.82 180.00 0.09 | | 13 54.16 -167.88 180.00 0.09 | | 14 109.29 -61.09 180.00 0.09 | | 15 123.12 -84.90 180.00 0.09 | | 16 64.66 -93.79 180.00 0.09 | | 17 113.09 108.59 179.89 0.09 | | 18 93.84 37.37 179.94 0.08 | | 19 90.00 -33.41 180.00 0.08 | | 20 131.95 90.00 90.75 0.08 | | 21 53.78 -144.59 180.00 0.08 | | 22 49.15 -43.11 180.00 0.08 | | 23 62.98 -56.60 180.00 0.08 | | 24 61.97 138.06 179.91 0.08 | | 25 96.77 59.47 179.90 0.08 | | 26 118.74 40.91 179.85 0.07 | | 27 100.14 57.97 179.86 0.07 | | 28 59.74 -139.98 180.00 0.07 | | 29 58.97 -141.14 180.00 0.07 | | 30 103.38 59.92 179.81 0.07 Many Thanks in advance for your kind help. THANK YOU