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Have you tried the RST tube (i.e. rapid clotting tube by BD which has thrombin in it to faciliatte clotting within 2- 3 mins)? We have found it very useful to obtain a very clear serum and it works very well with the hs-TnT assay (we use the Roche Cobas assay). Only disadvantage is the higher cost of the tube. best regards, Rita Horvath ________________________________ From: Clinical biochemistry discussion list [[log in to unmask]] On Behalf Of Steven John Mccann [[log in to unmask]] Sent: Thursday, 14 November 2013 2:59 AM To: [log in to unmask] Subject: Siemens troponin I ultra assay serum v plasma and ?flyers Hi there, Currently we use serum (greiner tubes) for routine work however it appears that around 1 in 300 of our positive TnI results on repeating on the same specimen come back negative. We use a cut-off of 20ng/L and currently repeat all samples above this to confirm the result. Negatives go straight out. The change noticed is beyond the 20ish% CV of the assay such that on some occasions results have been > 100ng/L but on repeat are <20ng/L. The samples are not spun prior to repeat testing. The cause is proposed to be microclots which affect us due to our rapid analysis. Do other users delay analysis to allow for complete clotting or use plasma instead? Do any other labs repeat positive TnI on the same sample if serum or plasma is used to check for ‘flyers’? Thank you for any replies. Steven Steven McCann FRCPath EurClinChem Consultant Clinical Biochemist and Clinical Biochemistry Lead Stepping Hill Hospital Poplar Grove Stockport NHS Foundation Trust SK2 7JE Tel No 0161 419 5619 Fax No 0161 419 5668 www.stockport.nhs.uk<http://www.stockport.nhs.uk/> www.nhslabmedservices.co.uk<http://www.nhslabmedservices.co.uk/> ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ --------------------------------------------------------------------------------------------- Illawarra Shoalhaven Local Health District, South East Sydney Local Health District and Sydney Children's Hospital Network (Randwick Campus) Confidentiality Notice This email, and the files transmitted with it, are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, you are not permitted to distribute or use this email or any of its attachments in any way. We also request that you advise the sender of the incorrect addressing. This email message has been virus-scanned. Although no computer viruses were detected, Illawarra Shoalhaven Local Health District, South East Sydney Local Health District and Sydney Children's Hospital Network (Randwick Campus) accept no liability for any consequential damage resulting from email containing any computer viruses. We care for our environment. Please only print this e-mail if necessary. ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/