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Dear Myrlene, the p1 images are only used once for the final realignment. Realignment can be only applied to images of the same modality (or type), thus p2 images (that represent white matter segmentations) should not be additionally used. Best, Christian On Tue, 29 Oct 2013 16:41:06 +0000, Myrlene Gee <[log in to unmask]> wrote: >Hi, > >I was looking at the longitudinal VBM8 job file, and in the final realignment step (corresponding to Realignment3 in the VBM8 manual, figure 4) the input files are the p1 images, entered twice. Should the second input file be the p2 images? > >I am using vbm8_r235. > >Thanks, >Myrlene