Hi Sjors, I get a strange error after iteration 13: Last message in the X.out file: 3/ 3 sec .........................................................~~(,_"> 3/ 3 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 4.015 degrees; offsets= 1.784 pixels Auto-refine: Angular step= 1.875 degrees; local searches= true Auto-refine: Offset search range= 4.014 pixels; offset step= 1.338 pixels Estimated required memory for expectation step= 0.128886 Gb, maximum allowed memory = 4 Gb. CurrentResolution= 32.4 Angstroms, which requires orientationSampling of at least 11.25 degrees for a particle of diameter 330 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 2654208 OrientationalSampling= 3.75 NrOrientations= 145 TranslationalSampling= 2.676 NrTranslations= 9 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 84934656 OrientationalSampling= 1.875 NrOrientations= 1160 TranslationalSampling= 1.338 NrTranslations= 36 ============================= Message in the X.err file: /relion-1.2_intel/bin/relion_refine_mpi: symbol lookup error: /home/lv70172/blaas/software/relion-1.2_intel/bin/relion_refine_mpi: undefined symbol: __libm_error_support /relion-1.2_intel/bin/relion_refine_mpi: symbol lookup error: /home/lv70172/blaas/software/relion-1.2_intel/bin/relion_refine_mpi: undefined symbol: __libm_error_support /relion-1.2_intel/bin/relion_refine_mpi: symbol lookup error: /home/lv70172/blaas/software/relion-1.2_intel/bin/relion_refine_mpi: undefined symbol: __libm_error_support and so on till the crash..... Any ideas? Thanks, Dieter -- Dr. Dieter Blaas Max F. Perutz Laboratories, Inst. Med. Biochem., Med. Univ. Vienna Dr. Bohr Gasse 9/3 A-1030 Vienna, Austria Tel. 0043 1 4277 61630 Fax 0043 1 4277 9616