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Hi, you could try dehydration in microdialysis buttons - this allows for slow gradual increase in PEG over few days and full control of other parameters, including lowering salt concentration. It was the only dehydration method that worked well for our large membrane protein: http://www.ncbi.nlm.nih.gov/pubmed/21822288 Described in more detail here: http://www.ncbi.nlm.nih.gov/pubmed/24059518