As Juergen has mentioned, both the T200 and the BioRad ProteOn (XPR36) are good options (we have both). The XPR36 is a higher throughput machine and can also be used for small molecule work (not quite as sensitive as the T200, but more sensitive than we had anticipated and certainly higher throughput). Good luck, tom Tom Peat Biophysics Group CSIRO, CMSE 343 Royal Parade Parkville, VIC, 3052 +613 9662 7304 +614 57 539 419 [log in to unmask] ________________________________ From: CCP4 bulletin board [[log in to unmask]] on behalf of Bosch, Juergen [[log in to unmask]] Sent: Tuesday, October 29, 2013 2:05 AM To: [log in to unmask] Subject: Re: [ccp4bb] Biacore/SPR BiaCore 3000 or if you can afford the T200. Proteon XPR36 would be a cheaper Option and should work as well. Jürgen On Oct 28, 2013, at 10:54 AM, Gang Dong wrote: We mainly measure protein-protein interactions (sometimes protein-small molecules). Thanks! _Gang From: Bosch, Juergen [mailto:[log in to unmask]] Sent: Monday, October 28, 2013 3:17 PM To: Gang Dong Cc: [log in to unmask]<mailto:[log in to unmask]> Subject: Re: [ccp4bb] Biacore/SPR Protein-protein interaction? protein-small molecule interactions ? Epitope mapping of mABs ? Could you specify what you would like to do, as different models are good for different things. Jürgen On Oct 28, 2013, at 10:08 AM, Gang Dong wrote: Dear all, Could anyone tell me your experience with Biacore (any models) or a similar SPR-based technology for measuring interaction kinetics? I also want to know the price ranges to discuss with our department/facility managers. Thanks, Gang ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu