Hi Oliver,
Have a look at the viral polyhedra work:
Coulibaly, F., et al., The molecular organization of cypovirus polyhedra. Nature, 2007. 446(7131): p. 97-101.
Coulibaly, F., et al., The atomic structure of baculovirus polyhedra reveals the independent emergence of infectious crystals in DNA and RNA viruses. Proceedings of the National Academy of Sciences of the United States of America, 2009. 106(52): p. 22205-22210.
Ji, X., et al., How baculovirus polyhedra fit square pegs into round holes to robustly package viruses. Embo Journal, 2010. 29(2): p. 505-514.
The crystals are typically small; wild type ~5um^3; recombinant ~10um^3. Because these are readily produced with very consistent unit cell, merging multiple crystals makes sense and allows best accumulation of completeness and redundancy. It is possible to collect complete data from a single, large crystal but with significant radiation damage. These crystals have unusually low solvent content ~22% so could be regarded as not generally representative.
Cheers,
Danny
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Oliver Zeldin
Sent: 11 October 2013 01:07
To: ccp4bb
Subject: [ccp4bb] Smallest crystal used for a whole dataset (at a synchrotron!)
Dear All,
I was wondering if anyone has a more up to date reference on the smallest crystal (fibrils not included!) that has been used to collect a whole dataset? Also, the smallest crystals used for a multi crystal approach? In both cases, not including any X-FEL structures.
I'm currently working off the citations in James Holton's 'A beginner's guide to radiation damage', but am sure that there must be a new record coming from the microfocus beamlines by now.
Cheers,
Oliver Zeldin
Brunger Lab
Stanford, CA
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