Is your gene of interest smaller than 750bp ?

Then I would synthesize the gene with IDT and optimize it for E.coli that's 139$

Jürgen

......................

Jürgen Bosch

Johns Hopkins Bloomberg School of Public Health

Department of Biochemistry & Molecular Biology

Johns Hopkins Malaria Research Institute

615 North Wolfe Street, W8708

Baltimore, MD 21205

Phone: +1-410-614-4742

Lab: +1-410-614-4894

Fax: +1-410-955-3655

http://lupo.jhsph.edu

On Sep 12, 2013, at 10:23, "Elias Fernandez" <[log in to unmask]> wrote:

Dear CCP4ers,
We’ve been struggling with little (nearly none) expression of our protein, in both E coli and with in vitro transcription/translation methods. It appears that our mRNA has high 2’ structure with a low dG (theoretically ~760kcal/mol). If this is indeed the source of our problem, are there any potential strategies to either disrupt the mRNA structure chemically (in E coli or in vitro) or with thermophile expression systems for expression at higher temperatures?
Regards,
Elias