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I've had good luck using the CCP4 DM program PARROT to improve poor initial MR maps with twinned data. (Based on some suggestions from this bb.) I think it works especially well if you have a lot of NCS symmetry, but it's worth a try, and doesn't take long. Cheers, _______________________________________ Roger S. Rowlett Gordon & Dorothy Kline Professor Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: [log in to unmask] On 9/23/2013 9:01 PM, Klaus Piontek wrote: > I would try phase improvement, especially since you have > two molecules per a.u. In other words averaging, solvent > flattning, histogram matching. The best way is to start at > low(er) resoltuion and extend to the highest resolution > vailable. The best criterium for success is a improved and > interpretable map. > I had quite some success with this appraoch with MR > solutions, but uninterpretable or difficult to build maps. > > Good luck, > > Klaus > > Dr. Klaus Piontek > Albert-Ludwigs University Freiburg > Institute of Organic Chemistry and Biochemistry, Room 401 H > Albertstrasse 21 > D-79104 Freiburg > Germany > Phone: ++49-761-203-6036 > Fax: ++49-761-203-8714 > Email: [log in to unmask] > > > --- the forwarded message follows ---