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I forgot to mention that in this case gene optimization and eukaryotic expression don't help. It's a product of the protein sequence itself. On Tue, Sep 24, 2013 at 10:29 AM, Katherine Sippel < [log in to unmask]> wrote: > Denizens of the bulletin board, > > I would like to tap into your vast knowledge of all things protein > expression. Is anyone aware of a cell line or even a > transformable/transfectable construct that produces high processivity > ribosomes. I.E. one that can translate to the end of difficult mRNA without > aborting prematurely? It doesn't have to be for E. coli. Yeast or Insect > cells would be acceptable too. > > Any help would be appreciated. > > Cheers, > Katherine > > -- > "Nil illegitimo carborundum"* - *Didactylos > -- "Nil illegitimo carborundum"* - *Didactylos