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Thread-Topic: tricky mr problem
Thread-Index: AQHOuKAU54fWlonFH0mSPYmePzZkPw==
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Date: Mon, 23 Sep 2013 22:01:32 +0100
Reply-To: RHYS GRINTER <[log in to unmask]>
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From: RHYS GRINTER <[log in to unmask]>
Subject: [ccp4bb] tricky mr problem
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Hi all,
I have been attempting to find a MR solution for a low resolution data set =
(3.9A), with pretty poor merging stats of a 22 strand membrane beta barrel =
I'm working on.
I've created a trimmed poly-alanine from a structure of 17% identity, that =
gives a solution with a Tfz of 14.3 with two molecules per asu (llg is arou=
nd 900). I'm guessing this in a genuine solution, but the map is too poor t=
o build into.
Does anyone have any advice as to proceed from here? It may be just a case =
of needing better resolution data to work with, but would this indicate tha=
t Selenomet derivative crystals won't be needed for this structure?
Cheers,
Rhys=
