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Parabolic is pretty crude and is the old (2.2 and earlier) default algorithm.  I would say that the gaussian or lorentzian fitting is probably better and which of those is more appropriate depends how you processed the spectrum. But likely they won't be too, too different in fitting, whereas the parabolic might.

Wayne

On 6 Aug 2013, at 14:56, Anne Gardner wrote:

Dear Wayne,

Thanks  Trillion....Yes it worked...Kindly confirm that when I click Recalc Fit button it asks various algorithms to calculate height...Parabolic, Guassian, and lorentzian. So which one is more appropriate. In NHSQC, it is parabolic so should I choose parabolic or any other?

Once again. Many thanks.
Best regards
Annie

From: Wayne Boucher <[log in to unmask]>
To: [log in to unmask]
Sent: Tuesday, 6 August 2013, 13:46
Subject: Re: Spin System Issue in sequence assignment

OK, it looks like this is because the peak heights are missing.  It might seem odd that it needs peak heights in the Protein Sequence Assignment dialog but it's using that to determine if the peak is positive or negative.

To get the peak heights filled in go to the Peak --> Peak Lists dialog in the Peak Table tab and select the Peak List and then select the relevant rows in the table (click on first row then shift click on last row).  Then click on the Recalc Fit button at the bottom (which re-calculates the position, the line width and the height).

Wayne

On 6 Aug 2013, at 11:56, Anne Gardner wrote:

Dear Wayne!

Hi, As in the last email, I can see whatever you asked. From  there further what should I do? I would greatly appreciate if you can guide me please!

Thanks

Best regards
Annie

From: Anne Gardner <[log in to unmask]>
To: CcpNmr software mailing list <[log in to unmask]>
Sent: Monday, 5 August 2013, 23:47
Subject: Re: Spin System Issue in sequence assignment

Dear Wayne,

Hello!  As you asked, so I have just checked that YES, I can see spin systems assigned during peak picking, in the Resonance Tab --> Spin Systems table in Assignment tab.  Secondly, yes in the assignment --> Protein sequence assign dialog box, in windows & spectra tab, query 13C is set.

Next Please?

With Kind regards
Annie
From: Wayne Boucher <[log in to unmask]>
To: [log in to unmask]
Sent: Monday, 5 August 2013, 21:02
Subject: Re: Spin System Issue in sequence assignment

Hello,

Are any spin systems shown in the Resonance --> Spin Systems table in the Assignments tab?

In the Assignment --> Protein Sequence Assignment dialog in the Windows & Spectra tab is there a "Query" 13C Window set?

Wayne

On 5 Aug 2013, at 20:53, Anne Gardner wrote:

Hello!

After peak picking in HNCA, HNCACB and CBCACONH spectra, Spin system list does not appear in a table, though I have assigned spin system in peak picking. I am working on stable version 2.3.0, both on Mac and PC windows. Normally after peak picking, the spin system list should appear in protein sequence assignment.

Annie

From: Simon Skinner <[log in to unmask]>
To: [log in to unmask]
Sent: Monday, 5 August 2013, 18:48
Subject: Re: Spin System Issue in sequence assignment

Hi 

Do you mean the spin system list is completely empty or that the type of spin system is not identified?

Simon


On Aug 5, 2013, at 7:21 PM, Anne Gardner wrote:

Hi
 
I am having problems with my project. I’ve peak picked and initialised my Nhsqc and peak picked my HNCA, HNCACB and CBCACONH. My problem is when I start to sequence assign. My spin systems don’t appear in the table. All this was done on the Mac version of CCPN.
 
I’ve done exactly the same thing on the PC version but just selecting 3 spin systems for speed (as a check) and my spin systems come up fine in sequence assign. Is there a quick fix? I don’t want to have to peakpick three 3D spectra again! I’ve tried exporting peaks and sequences etc but have no luck reimporting them into a new project.
 
Thanks

Annie