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Hi Annie,

Do you have two separate windows for the query and the reference spectrum? 

Secondly, have you used the pick and assign root resonances to link the HSQC to the HNCACB, CBCACONH and HNCA, such that all linked peaks have the same spin system assignment?

Simon
On Aug 6, 2013, at 12:47 AM, Anne Gardner wrote:

Dear Wayne,

Hello!  As you asked, so I have just checked that YES, I can see spin systems assigned during peak picking, in the Resonance Tab --> Spin Systems table in Assignment tab.  Secondly, yes in the assignment --> Protein sequence assign dialog box, in windows & spectra tab, query 13C is set.

Next Please?

With Kind regards
Annie
From: Wayne Boucher <[log in to unmask]>
To: [log in to unmask]
Sent: Monday, 5 August 2013, 21:02
Subject: Re: Spin System Issue in sequence assignment

Hello,

Are any spin systems shown in the Resonance --> Spin Systems table in the Assignments tab?

In the Assignment --> Protein Sequence Assignment dialog in the Windows & Spectra tab is there a "Query" 13C Window set?

Wayne

On 5 Aug 2013, at 20:53, Anne Gardner wrote:

Hello!

After peak picking in HNCA, HNCACB and CBCACONH spectra, Spin system list does not appear in a table, though I have assigned spin system in peak picking. I am working on stable version 2.3.0, both on Mac and PC windows. Normally after peak picking, the spin system list should appear in protein sequence assignment.

Annie

From: Simon Skinner <[log in to unmask]>
To: [log in to unmask]
Sent: Monday, 5 August 2013, 18:48
Subject: Re: Spin System Issue in sequence assignment

Hi 

Do you mean the spin system list is completely empty or that the type of spin system is not identified?

Simon


On Aug 5, 2013, at 7:21 PM, Anne Gardner wrote:

Hi
 
I am having problems with my project. I’ve peak picked and initialised my Nhsqc and peak picked my HNCA, HNCACB and CBCACONH. My problem is when I start to sequence assign. My spin systems don’t appear in the table. All this was done on the Mac version of CCPN.
 
I’ve done exactly the same thing on the PC version but just selecting 3 spin systems for speed (as a check) and my spin systems come up fine in sequence assign. Is there a quick fix? I don’t want to have to peakpick three 3D spectra again! I’ve tried exporting peaks and sequences etc but have no luck reimporting them into a new project.
 
Thanks

Annie