OK, that sounds like an out and out bug, it's not supposed to try and map 15N to 13C. I have an HNCA with the same dimension ordering as you and it's working ok for me, so it has Dim2 being mapped to Dim3 for the 15N. Can you send me just the project (off list) so I can try to reproduce this.

Thanks, Wayne

On 6 Aug 2013, at 09:34, Tom Carruthers wrote:

Sorry Wayne let me clarify,

It was an N-HSQC and a 3D HNCA. I wanted to re-reference the N-HSQC to the HNCA (so opposite to your example). My HNCA is an nmrPipe file that is ordered as a series of 1H-C13 planes (i.e. Dim1 was 1H, Dim2 was 13C and Dim3 was 15N). I have started to order my spectra in this way as it seems to make analysis display the spectra quicker.

If I select the HNCA peak second the re-reference window does turn up and does allow me to re-reference the N-HSQC to the HNCA, but it doesn't give the option of which dimension to reference to - so it defaults to referencing dim1 to dim1 (1H-1H correct) and Dim2 to Dim2 (15N to 13C - incorrect). This is unlike the case you describe where you are re-referencing a 3D using a fixed 2D experiment - which works as expected.

Hope that clears up my query.

Tom

On 6/08/13 6:01 PM, Wayne Boucher wrote:
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So this is an HSQC and a 3D Noesy or something? If you want to keep the 2D fixed and re-reference the 3D to that and (say) there is a repeated 1H dimension in the 3D then when you first open up that dialog it will map the 2D 1H to the first 3D 1H but if you double click in the "Reference by Peak" dialog on the second 1H row in the "Fixed Dim" cell it should pop up an option to make that the dimension that the 2D 1H gets mapped to.  At least that seems to work for me.

Doing many spectra in one go is a trickier issue because it would need an upgraded dialog, I will look into that.

I guess you noticed that it is the last peak selected that is fixed and the other peak that is re-referenced.

Wayne

On 6 Aug 2013, at 07:33, Tom Carruthers wrote:


Hi there,

I think I may have found an inconvenient feature of the 'Reference By Peak' window {R:Peak:Re-reference to this peak}. If you chose to reference a 3D to a 2D spectrum it gives you the option to choose which dimension to match to which, however if you want to reference a 2D to a 3D it automatically assumes that 2D.F1=3D.F1 and 2D.F2=3D.F2 (which in my case is not true). This happens even if the dimensions are not compatible - I lost my 2D HSQC as it tried to shift the carrier to match the CA region... This is a pain as I have referenced all my 3D's to each other and now I need to re-reference them all or do the 2D reference manually.

Also, I note that you can't reference more than 2 spectra at one time (i.e. you can't reference an entire data set at once). Although I know this requires more programming at your end, it'd be nice!

Tom Carruthers