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Dear Sjors, I have a multi-part complex in which one region is always fuzzy. Is there a way to have RELION do 2D alignment focused on this fuzzy region? Reggie -- Reginald McNulty, Ph.D. Postdoctoral Research Associate The Scripps Research Institute Johnson Lab Department of Integrative Structural and Computational Biology 10550, N. Torrey Pines Road, MB-31 La Jolla, California 92037 Telephone: 858-784-8652 Cell: 949-436-1263 Email: [log in to unmask]