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But when it sits and crystallizes it is cleaner - unless some opportunistic fungal contamination helps you trim off those nasty loops that you would have omitted anyhow from your model.

Jürgen

P.S. I like kangaroo's in particular when served with red wine and medium rare next to some garlic mashed potatoes

On Aug 22, 2013, at 2:17 PM, Ed Pozharski wrote:

On Thu, 2013-08-22 at 13:58 -0400, Bosch, Juergen wrote:
well if we hit the timer after lysis, say via cell disruptor then I
have my eluted protein in less than 1 hour, including 40 minutes batch
binding.

then proceeds to wait six weeks for crystals to appear... :)

Cheers,

Ed.

PS.  There are many ways to skin a kangaroo and ultimately indeed all
things serve the Beam.

--
"I'd jump in myself, if I weren't so good at whistling."
                              Julian, King of Lemurs


......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab:      +1-410-614-4894
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http://lupo.jhsph.edu