Hi Kevin et al.
Buccaneer IS a great program and I like it very much.
I have a testcase here for my X-ray class where a trimer is in the asymmetric unit, however all copies are different (not in sequence, but in space - think of Calmodulin for example).
There is no option to force Buccaneer to not use NCS right ? In this particular case the autobuilding is not performing as well as it could if it were not to use NCS.
I assume from the command line -ncsbuild is turning it on, however does not including this keyword turn off NCS building, or is your program so smart that it overrides the user wishes because it knows better ?
Thanks,
Jürgen
P.S. I can provide you with the SeMet phased dataset and the sequence for you to play with.
......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-2926
http://lupo.jhsph.edu
