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Hi Omi
Did you test them for diffraction? you might want to keep screening broad for better crystals.  You could try to add K/NaSCN, KI or KNO3 as an additive (50 mM) to you protein mix and go from there
best Preben
On 8/23/13 4:56 AM, zhuqing ouyang wrote:
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Dear all, I am trying to crystallize a protein complex, the initial screening result shows that several hints look like microcrystals. see pictures attached. The conditions are very similar:
1. 0.2 M NaI, 20% 3350
2. 0.2 M KI, 20% 3350
3. 0.2 M NaSCN, 20% 3350
4. 0.2 M KSCN, 20% 3350
5. 0.2 M NaNO3, 20% 3350
6. 0.2 M KNO3, 20% 3350

But I can not get bigger crystals around those conditions. Any comments or suggestions would be appreciated.
best
omi


-- 
J. Preben Morth, Ph.D
Group Leader
Membrane Transport Group
Nordic EMBL Partnership
Centre for Molecular Medicine Norway (NCMM)
University of Oslo
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