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Hi Omi, Just to add to Preben's suggestion, something that jumps out from the conditions you list - those are all fairly chaotropic anions. It might also be worth looking into other anions from the same end of the Hofmeister series (i.e. bromide), and possibly chaotropic cations as well. Shane Caldwell McGill University On Fri, Aug 23, 2013 at 2:32 AM, jens Preben Morth <[log in to unmask]>wrote: > Hi Omi > Did you test them for diffraction? you might want to keep screening broad > for better crystals. You could try to add K/NaSCN, KI or KNO3 as an > additive (50 mM) to you protein mix and go from there > best Preben > > On 8/23/13 4:56 AM, zhuqing ouyang wrote: > > Dear all, I am trying to crystallize a protein complex, the initial > screening result shows that several hints look like microcrystals. see > pictures attached. The conditions are very similar: > 1. 0.2 M NaI, 20% 3350 > 2. 0.2 M KI, 20% 3350 > 3. 0.2 M NaSCN, 20% 3350 > 4. 0.2 M KSCN, 20% 3350 > 5. 0.2 M NaNO3, 20% 3350 > 6. 0.2 M KNO3, 20% 3350 > > But I can not get bigger crystals around those conditions. Any comments > or suggestions would be appreciated. > best > omi > > > -- > J. Preben Morth, Ph.D > Group Leader > Membrane Transport Group > Nordic EMBL Partnership > Centre for Molecular Medicine Norway (NCMM) > University of Oslo > P.O.Box 1137 Blindern > 0318 Oslo, Norway > > Email: [log in to unmask] > Tel: +47 2284 0794 > http://www.jpmorth.dk > >