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Hi Reggie, Not really, although you could try to provide a 2D mask and then use very strict local angular searches (effectively prohibiting the particles to move at all). This goes beyond the GUI though: you'll need to add the corresponding command-line arguments yourself. You could try the 3D refinement GUI to see what those arguments should be (they're the same for 2D and 3D). HTH, S > Dear Sjors, > > I have a multi-part complex in which one region is always fuzzy. Is there > a way to have RELION do 2D alignment focused on this fuzzy region? > > Reggie > > -- > Reginald McNulty, Ph.D. > Postdoctoral Research Associate > The Scripps Research Institute > Johnson Lab > Department of Integrative Structural and Computational Biology > 10550, N. Torrey Pines Road, MB-31 > La Jolla, California 92037 > Telephone: 858-784-8652 > Cell: 949-436-1263 > Email: [log in to unmask] -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres