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Hello Nazli, as I said, if you did these steps correctly, you should not have encountered a problem. Two more remarks: one, in order for this to work, your functionals need to be in register with each other (cf. realignment), and two, do not use the normalize button (cf. unified segmentation). One way to find out which volumes are the offending ones is to simply do something like vols = spm_read_vols(spm_vol(spm_select(Inf, 'image'))); and select all normalized images. The following error will list the orientation of all images so you can find the offending/different one. Good luck, Marko nazi mohajeri wrote: > Dear Marko, > I have first coregistered each sunject's functional to its anatomical > image (reference image= anatomical image, source image= first volume and > other images= rest of the volumes) then in normalization, I selected > each subject's anatomical image as source image and the volumes of its > functional image as images to write. > Is there anything incorrect in this procedure that may cause problem in > orientation ?? > Thanks, > Nazli > > > Hello Nazli, > > > I want to apply one and two sample t-test on zFC maps. Matlab meets > error of mismatch in orientation. > > I have done normalization but the images do not have same > orientations. because first, I have coregistered each subject's > functional data to its sturactural image and then normalized it to MNI > by using that. > > If I understood you correctly, what you have done (coregister individual > functional to individual anatomical, normalize that and apply parameters > to coregistered functionals) is one of the recommended ways of doing > things. If you have done this correctly, then the orientations should be > identical. One common source of error that you might want to look into > is that it is easy to forget to apply the reorientation from > coregistration to just one functional image, which will then lead to a > different orientation in normalized space. > > Cheers, > Marko > > -- > ____________________________________________________ > PD Dr. med. Marko Wilke > Facharzt für Kinder- und Jugendmedizin > Leiter, Experimentelle Pädiatrische Neurobildgebung > Universitäts-Kinderklinik > Abt. III (Neuropädiatrie) > > > Marko Wilke, MD, PhD > Pediatrician > Head, Experimental Pediatric Neuroimaging > University Children's Hospital > Dept. III (Pediatric Neurology) > > > Hoppe-Seyler-Str. 1 > D - 72076 Tübingen, Germany > Tel. +49 7071 29-83416 > Fax +49 7071 29-5473 > [log in to unmask] <mailto:[log in to unmask]> > > http://www.medizin.uni-tuebingen.de/kinder/epn/ > ____________________________________________________ > > -- ____________________________________________________ PD Dr. med. Marko Wilke Facharzt für Kinder- und Jugendmedizin Leiter, Experimentelle Pädiatrische Neurobildgebung Universitäts-Kinderklinik Abt. III (Neuropädiatrie) Marko Wilke, MD, PhD Pediatrician Head, Experimental Pediatric Neuroimaging University Children's Hospital Dept. III (Pediatric Neurology) Hoppe-Seyler-Str. 1 D - 72076 Tübingen, Germany Tel. +49 7071 29-83416 Fax +49 7071 29-5473 [log in to unmask] http://www.medizin.uni-tuebingen.de/kinder/epn/ ____________________________________________________