Dear Vivoli,

Thanks for your kind inputs. The case of Proline is already taken care of during cloning. I should have pointed this, but thanks that you did mentioned it.

Thanks again for inputs.

With best regards
Rajesh

On Sun, Jul 21, 2013 at 12:34 PM, K Singh <[log in to unmask]> wrote:

Dear All,
I have expressed fusion protein that is expressed in the following format

Fusion partner - Enterokinase cleavage site - Protein of interest.

somehow enterokinase is not doing its job though following manufacturers recommendations. any inputs like what protocol worked.

Many thanks for your inputs

Kris