Hi expert, I met some questions in hippocampus segmentation,the details are as follows. First,I use spm to normalise the sMRI.Make the size of volex is 1*1*1. second,I use bet and first of FSL to segmentation the hippocampus.The commands are : for filename in *.img;do fname='${FSLDIR}/bin/remove_ext ${filename}' bet ${fname}.img ${fname}_brain -f 0.2 -g 0 fname2=${fname}_brain run_first_all -i ${fname2} -b -d -s L_Hipp,R_Hipp -o ${fname2} done Sometimes,I can get the right result,but sometimes I cannot get the right result.If I do not use the spm,I can get the right result.I want to know the reasons. Thank you very much, Best wishes Wily