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Hi everyone, I now meet some problems when trying to solve structure.Space group is P6422, and Mathews function shows there are 4 molecules in one asymmetry unit. However, Phenix-autobuild shows only 2 molecules in on one asymmetry unit, after refinement, Rfree=20%,(resolution is 2.8A). Although AA fit the map well, there are still some green and red density. I wonder if anyone met this problem before? Any suggestion is welcome. Best, Jiang Yan