Hi Wayne,

Thank you for the suggestion. But I think that maybe not the problem, cause I have only one set of resonance. This is what I did, is there anything wrong?

1. Open the spectra (I opened Nhsqc, cbcaconh, hncacb)

2. Manually peak picked the Nhsqc.

3. Assignment->Initialise Root Resonace (selected the sidechains and confirm)

4. Assignment->Pick & Assign form Root (adjust the tolerence 0.2ppm for N15 and 0.02ppm for proton)->Pick all & Assign Root Resonance

5. Molecule->Add seqence (To build the protein molecule)

6. Window->Clone (To clone a triple resonance window, say the former one is window3, the new one is window4)

7. Assignment->Protein sequence Assignment (query window use window3 and cbcaconh, match window use window4 and hncacb. leave {options}untouched)

8. in the {spin systems tab}, when I click on one spin system, I could see the query window works ok, but the match window doesn't show any match strips. It seemed that the match window had found something, since when I click on differenct spin systems, different numbers of strips were opened. But nothing is in the strip because every strip in the match window is showing the middle of proton chemical shift, lowest N15 chemical shift and the right C13 chemical shift.

I've attached a screenshot, hope I could do that in a maillist and you can see it.

Thank you very much!

Best Regards,

Xuehui

From: Wayne Boucher <[log in to unmask]>
To: [log in to unmask]
Sent: Friday, May 24, 2013 5:13 PM
Subject: Re: Problem with the Assignment: Protein Sequence Assignment

Hello,

Tim suggests to check that the triple resonance peaks in both spectra are
assigned to amide root resonances and that both spectra are assigned to
the same set of resonances.

Wayne

On Wed, 22 May 2013, Xue Liu wrote:

> Hi
> I am a new user of the CCPNMR and am trying a backbone assign job with it now. I think i've followed
> every step of the tutorial （http://www.ccpn.ac.uk/software/tutorials/analysis-beginners）, but I got
> this problem with the Protein Sequence Assignment. When I click on the spin systems, I should get the
> matching strips on the Match Window according to the turtorial, but I get nothing but hollow strips
> showing the middle of proton chemical shift range and N15 set to the lowest frequency with the C13
> showing the Ca and Cb ruler positions.
> I 've tried several times and I even tried the windows version. I also checked the mailing list posts
> for answers but I did not find anything.
> By the way, I am using CBCACONH in the query window and HNCACB in the match window.
> Can anyone give some suggestion on this, thanks a lot.
>
>
> Best Regards,
>
> Xuehui
>
>