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Hello, The peak dimensions and how they are treated is separate from how the window axes. So the peak dimensions are fixed by how the data is stored on disk. When Analysis reads the Bruker acqu*/proc* files it determines what order the various isotopes occur on disk (hopefully correctly!). So if it thinks the 15N is the second one then that is what the Bruker files are (supposedly) claiming. Could the dimensions have been transposed during processing? The Protein Sequence Assignment tool is exactly meant for experiments like HNCA. Those experiments should be listed in both the Query and Match tables if an appropriate 13C Window is selected in the pulldown menu just above the tables. Wayne On Sun, 12 May 2013, Raphael Sayegh wrote: > Dear CCPNMR users, > > I'm having some troubles to get the proper dimensions (F1=H; F2=13C; > F3=15N) in a HNCA experiment processed in Topspin. > > Before opening the spectrum, I have to create a window with 15N in F3 > and 13C in F2 dimensions to visualize it with 15N in the Z-axis. > Otherwise I would get 13C in F3 (z-axis) and 15N in F2 (y-axis). > > However, the 15N dimension keeps labeled as F2 in my peak lists. The > peaks were picked using the "pick and assign from roots" tool. I suspect > that this creates troubles for the sequence assignment with the > Assignment:Protein Sequence Assignment tool, because this tool doesn't > list spectral windows for 13C, only for 1H...! > > Does anyone know how to change the 15N dimension label in the peak list? > > I really appreciate any help you can provide. > > Best regards, > Raphael >