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Dear Yan: I had a similar problem with the crystal(thin, thread diffraction in one direction ). Many try with the crystal optimization failed. However using micro-focus beamline with a small oscillation angel (0.1- 0.2 degree) I could collect a full data set and successfully processed with XDS. Best Xiaohu On May 29, 2013, at 3:18 AM, 姜艳 wrote: > Dear professors, > I get my crystal in 0.1M Tris, PH7.5, 200mM (NH4)2SO4, and 20% PEG3350, however, it is very thin. From one side, the diffraction is perfect, about 2.2A, but from the other side, diffraction is too bad, the spots look like a thread! Process cannot be done by HKL2000. > As top guns of this field, could you give me some suggestion to make the crystal thicker? I will be grateful for your kind help. > Best, > Jiang Yan > > Institute of Biophysics, Chinese Academy of Sciences > Beijing, Chaoyang District > > --------------------------------------------------------------- Xiaohu Guo, Ph.D student Department of Cell and Molecular Biology, Uppsala University Uppsala Biomedical Center (BMC) Box 596 S-751 24 Uppsala Sweden Phone: +46 765826578