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I think there are some mitegen loops that are flat. They will probably be useful for your crystals given what you say about the 2.2 Å anisotropic diffraction, which is probably caused by deformation of your plates in "conventional" loops. Some others on the list may have a more specific recommendation about this brand of loops. Also, you may want to check the mitegen website for specific products and applications. James On May 28, 2013, at 7:18 PM, 姜艳 wrote: > Dear professors, > I get my crystal in 0.1M Tris, PH7.5, 200mM (NH4)2SO4, and 20% PEG3350, however, it is very thin. From one side, the diffraction is perfect, about 2.2A, but from the other side, diffraction is too bad, the spots look like a thread! Process cannot be done by HKL2000. > As top guns of this field, could you give me some suggestion to make the crystal thicker? I will be grateful for your kind help. > Best, > Jiang Yan > > Institute of Biophysics, Chinese Academy of Sciences > Beijing, Chaoyang District > >