Hi Alex,

If you do not have access to HPLC equipment, another alternative is gel-purification using a PAGE setup under denaturing (urea) conditions. This has the advantage of being fairly simple and effective for a range of transcripts, but you will need a fairly large gel tank system to get good resolution. Ke and Doudna 2004 review in Methods is an excellent starting point for learning about practical issues involved in the crystallisation of protein/RNA complexes and purification of RNA for this purpose.

Hope this helps,

Eugene

On 20 May 2013 15:20, A K <[log in to unmask]> wrote:

Dear all,

I have a crystallization-related question. I am going to co-crystallize protein with RNA. I ordered a short (10 mer and 14 mer) RNA from Dharmacon but did not choose the purification option while ordering them due to the budget issues. How critical is to HPLC purify them before setting drops? Aren't RNA synthesis protocols reliable enough nowadays for such short RNAs?

Thanks,

Alex

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Dr Eugene Valkov

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