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Dear FSL experts I'd better check my following steps... After TBSS analysis, I'd like to obtain a volume that shows me only differences between radial diffusivity and FA significant clusters. First, I thresholded my corrp images: fslmaths FA_corrp_image -thr 0.95 FA_corrp_image_thr0.95 and fslmaths RD_corrp_image -thr 0.95 RD_corrp_image_thr0.95 Then, I've subtracted fslmaths FA_corrp_image_thr0.95 -sub RD_corrp_image_thr0.95 new_image_FA_sub_RD fslmaths RD_corrp_image_thr0.95 -sub FA_corrp_image_thr0.95 new_image_RD_sub_FA Thanks for your help Gayane