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Hi - most likely this means your input image includes negative values. All the -mas is doing is zeroing voxels not in the mask. Steve. On 19 Apr 2013, at 03:37, Armin Biller <[log in to unmask]> wrote: > hi, > > i'm a little worried about fslmaths which introduces negative values to the data after "fslmaths -mas" (extracting tumor VOIs). how comes? > > any idea is very much appreciated! > > all the best, > armin > --------------------------------------------------------------------------- Stephen M. Smith, Professor of Biomedical Engineering Associate Director, Oxford University FMRIB Centre FMRIB, JR Hospital, Headington, Oxford OX3 9DU, UK +44 (0) 1865 222726 (fax 222717) [log in to unmask] http://www.fmrib.ox.ac.uk/~steve ---------------------------------------------------------------------------