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Hi Amanda, This is an interesting question. I wonder if the partly gelatinized starch grains have a slightly different specific gravity to less damaged starch, meaning they remain in the pellet. Can you find out what s.g. they float at? It may mean that certain samples should be prepped twice at two different s.g. to obtain all starch. Regards, Sheahan Bestel Post-doctoral fellow, University of Toronto at Mississauga Sent from my iPhone On 2013-04-23, at 1:47 AM, "Amanda Henry" <[log in to unmask]> wrote: > Hello, > > I'm wondering if anyone on the listserv has used sodium polytungstate to try to separate partially gelatinized starches from sediment samples. We've been doing some experiments with modern materials and have found that SPT at 1.8g/ml seems to preferentially float native starches, leaving the partially gelatinized ones in the pellet. Has anyone else run into this problem? Any suggestions for a better method? > > Many thanks in advance, > Amanda > > > Amanda Henry, PhD > Independent Research Group Leader > Max Planck Institute for Evolutionary Anthropology > Leipzig, Germany > http://www.eva.mpg.de/plants