You need to apply the coregistration parameters to the CBF image before normalization.

Best Regards, Donald McLaren
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Research Fellow, Department of Neurology, Massachusetts General Hospital and
Harvard Medical School
Postdoctoral Research Fellow, GRECC, Bedford VA
Website: http://www.martinos.org/~mclaren
Office: (773) 406-2464
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On Mon, Mar 4, 2013 at 9:30 PM, Bin-Ke YUAN <[log in to unmask]> wrote:

Dear Wang,

Thanks for your reply! If I correctly understand you:  Firstly, the control image was corregistered with the corresponding T1 image;Secondly,the T1 image were normalized to MNI space;Finally,the CBF images (1.72x1.72x3) were spatially normalized and re-sampled (2x2x2 mm) using the normalization parameters estimated during the previous T1 normalization. Is the procedure right or suitable?

sincerely, 

Bin-Ke

2013/3/5 Ze Wang <[log in to unmask]>

Hi Bin-Ke,
      GE ASL sequence produces mean control image (regular FSE image) and mean CBF map (or the mean perfusion difference map if the scale is not within 0-100). To register cbf map to T1, you'd better use the mean control image rather than the cbf map, then apply the same transformation to the cbf map.   This process is the same for any vendor.  You can find more information in the manual available in
cfn.upenn.edu/~zewang/ASLtbx.php

Best
Ze

On 3/4/2013 5:51 AM, Bin-Ke YUAN wrote:

Dear all:

 I used unified segmentation to perform normalization of my CBF images and found the normalization effect was bad (images of unnormalized and normalized were attached). I have done the corregister before normalization.

 The pulse sequence of ASL are 3DASL (no contrast,GE healthcare MR-750 3.0 T scanner) and the CBF images were got using the 3DASL functiontool of the scanner.

 Any suggestion / advice will be very welcome.

 Many thanks in advance,

 Bin-Ke

-- 
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Ze Wang, Ph. D
http://cfn.upenn.edu/~zewang
Research Assistant Professor of Biomedical Engineering,
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Perelman School of Medicine,
University of Pennsylvania,
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