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Dear Bin-Ke, Which template have you used? You wrote you did a coregistration before. Did you coregister the ASL images with an T1 image and segmented this one or what exactly was your procedure? You could also try to normalize the CBF maps to a PET template and create your own population based template out of that and re-normalize your scans. Some ASL sequences are providing also a PD image. You may want to try out a normalization on that one, as well. Good luck, Karsten Fra: Bin-Ke YUAN <[log in to unmask]> Svar til: Bin-Ke YUAN <[log in to unmask]> Dato: 10Montag, 4. März 2013 11:51 Til: <[log in to unmask]> Emne: [SPM] Normalization of ASL images Dear all: I used unified segmentation to perform normalization of my CBF images and found the normalization effect was bad (images of unnormalized and normalized were attached). I have done the corregister before normalization. The pulse sequence of ASL are 3DASL (no contrast,GE healthcare MR-750 3.0 T scanner) and the CBF images were got using the 3DASL functiontool of the scanner. Any suggestion / advice will be very welcome. Many thanks in advance, Bin-Ke