Dear members of ccp4bb,
I need some help with the refinement of my structure of a variant of mRFP (monomer red fluorescent protein, sequence in attachment). I have done molecular replacement with phaser with model 2VAD of protein database. Then i have done some model building phenix.autobuild.
(2 pdb's (overall...), freeR flags and log file attached) When i refine with phenix.refine my structure i get a R-value of 0,42 which is still way too high. (redfluorescent protein.pdb, .mtz and logfile attached) When i look at the structure in coot i find
many unmodelled blobs and many outliers in density analysis and rotamer analysis. The problem is that there are so many problems with my structure, that i dont know where to begin. Could you try some refinement for me, because this is first structure that
i need to solve as a student and i dont have too many experience with it.
Greetings,
Tom
<overall_best.pdb><exptl_fobs_phases_freeR_flags.mtz><overall_best_placed.pdb><redfluorescentprotein_refine_10.pdb><redfluorescentprotein_refine_10.mtz><mRFPsequentiezondertag.fasta><redfluorescentprotein_refine_10.log><AutoBuild_run_6_1.log>