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Dear Tom, As far as I know you are the first person who sent his/her data that are supposed to be _private_ to 1000+ CCP4BB subscribers. The person who suggested you send this type of message to CCP4BB board played a very cruel practical joke on you. To CCP4BB support staff: Is it possible to block emails with 1+MB attachments? Thank you, Petr ------------------------ Prof. Petr Leiman EPFL BSP-415 CH-1015 Lausanne Switzerland On Mar 27, 2013, at 5:22 PM, Tom Van den Bergh wrote: Dear members of ccp4bb, I need some help with the refinement of my structure of a variant of mRFP (monomer red fluorescent protein, sequence in attachment). I have done molecular replacement with phaser with model 2VAD of protein database. Then i have done some model building phenix.autobuild. (2 pdb's (overall...), freeR flags and log file attached) When i refine with phenix.refine my structure i get a R-value of 0,42 which is still way too high. (redfluorescent protein.pdb, .mtz and logfile attached) When i look at the structure in coot i find many unmodelled blobs and many outliers in density analysis and rotamer analysis. The problem is that there are so many problems with my structure, that i dont know where to begin. Could you try some refinement for me, because this is first structure that i need to solve as a student and i dont have too many experience with it. Greetings, Tom <overall_best.pdb><exptl_fobs_phases_freeR_flags.mtz><overall_best_placed.pdb><redfluorescentprotein_refine_10.pdb><redfluorescentprotein_refine_10.mtz><mRFPsequentiezondertag.fasta><redfluorescentprotein_refine_10.log><AutoBuild_run_6_1.log>