The first report of shooting a protein crystal at a synchrotron (I
think) was in 1976:
http://www.pnas.org/content/73/1/128.full.pdf
that was rubredoxin

The first PDB file that contains a "SYNCHROTRON=Y" entry is 1tld
(trypsin), which was deposited in 1989:
http://dx.doi.org/10.1016/0022-2836(89)90110-1
But the structure of trypsin was arguably already "solved" at that time.

Anomalous diffraction was first demonstrated by Coster, Knoll and Prins
in 1930
http://dx.doi.org/10.1007/BF01339610
this was 20 years before Bijvoet.  But not with a synchrotron and
definitely not with a protein

The first protein to be solved using anomalous was crambin in 1981:
http://dx.doi.org/10.1038/290107a0
but this was not using a synchrotron

The first demonstration of MAD on a protein at a synchrotron was a Tb
soak of parvalbumin in 1985
http://dx.doi.org/10.1016/0014-5793(85)80207-6
but one could argue that several parvalbumins were already known at that
time.

The first MAD structure from native metals was cucumber blue copper
protein (2cbp) in 1989
http://dx.doi.org/10.1126%2Fscience.3406739

The first "new" structure using MAD, as well as the first SeMet was
ribonuclease H (1rnh) in 1990
http://dx.doi.org/10.1126/science.2169648

If anyone knows of earlier cases, I'd like to hear about it!

-James Holton
MAD Scientist

On 3/13/2013 7:38 AM, Alan Cheung wrote:

Hi all - i'm sure this many will know this : when and what was the

first protein structure solved on a synchrotron?

Thanks in advance

Alan