 |
 |
The disulfide bond is intramolecular. I do not have reasons to believe it is cross linking my protein
On Wed, 6 Feb 2013 22:16:36 -0500, Jacob Keller wrote:
Couldn't you just run reducing/non-reducing SDS-PAGE lanes and see the difference?
JPK
On Wed, Feb 6, 2013 at 11:10 AM, Yuri Pompeu <[log in to unmask]> wrote:
Dear All,
I am trying to probe the existence of a disulfide bond on the surface of my protein.
I have attempted Ellmanīs and my results were not as clear as I would have hoped for.
I am not a sulfur/cysteine chemist and would appreciate the advice on what experiments to try!
Thanks a bunch
YAP
--
*******************************************
Jacob Pearson Keller, PhD
Postdoctoral Associate
HHMI Janelia Farms Research Campus
email: [log in to unmask]
*******************************************
-- Yuri Pompeu