I never done such experiments but N-Ethylmaleimide labeling and Mass-Spec could be the solution.

Le 6 févr. 2013 à 17:10, Yuri Pompeu <[log in to unmask]> a écrit :

Dear All,
I am trying to probe the existence of a disulfide bond on the surface of my protein.
I have attempted Ellman´s and my results were not as clear as I would have hoped for.
I am not a sulfur/cysteine chemist and would appreciate the advice on what experiments to try!
Thanks a bunch
YAP

Xavier Brazzolotto, PhD
Département de Toxicologie
Institut de Recherche Biomédicale des Armées
24 Av des Maquis du Grésivaudan
38702 La Tronche Cedex
France