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I never done such experiments but N-Ethylmaleimide labeling and Mass-Spec could be the solution. Le 6 févr. 2013 à 17:10, Yuri Pompeu <[log in to unmask]> a écrit : > Dear All, > I am trying to probe the existence of a disulfide bond on the surface of my protein. > I have attempted Ellman´s and my results were not as clear as I would have hoped for. > I am not a sulfur/cysteine chemist and would appreciate the advice on what experiments to try! > Thanks a bunch > YAP Xavier Brazzolotto, PhD Département de Toxicologie Institut de Recherche Biomédicale des Armées 24 Av des Maquis du Grésivaudan 38702 La Tronche Cedex France