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I've just read that today (even if it is NMR). http://www.nature.com/nmeth/journal/v9/n8/full/nmeth.2033.html /E. coli/ or insects cells are so easy to grow that you should be able to do your own cell extrats easily. Xavier Le 27/02/2013 19:25, Theresa Hsu a écrit : > Dear all > > Out of curiosity, are cell free expression systems using E. coli or insect cell extracts useful for membrane protein expression-crystallization? What are the costs associated with these systems? > > Thank you. > > Theresa