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Dear Mark,

I have had quite a lot of success by adding small molecules during cell lysis to aid in solubility. From screening 100+ condition we found trehalose, betaine and mannitol to be the standouts with aiding in solubility.

Here is the relevant paper: http://www.ncbi.nlm.nih.gov/pubmed/23285060 

David


On Tue, Feb 26, 2013 at 2:08 AM, Mark J van Raaij <[log in to unmask]> wrote:
Dear All,

We are considering trying additives to bacterial cultures to see if they improve solubility of some proteins we express: L-arginine, glycyl-glycine, sorbitol and betaine. I would be glad to receive recommendations and experiences, also if they haven't worked.
Side-question: should we get cell culture-certified compounds, or would "normal", much more economical ones suffice? (i.e. are they likely to contain side-products that would affect bacterial growth?).

Greetings,

Mark

Mark J van Raaij
Lab 20B
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://www.cnb.csic.es/~mjvanraaij




--
David J. Leibly
Graduate Student, Yeates Lab
Dept. Chem & Biochem
Univ. of California, Los Angeles
Boyer Hall 269B
(310) 825-8901