 |
 |
Try using dioxane as an additive. I'd suggest adding 5- 15% dioxane into your well solution. This should inhibit nucleation resulting in fewer, larger crystals. On 25/02/13, Felix Frolow <[log in to unmask]> wrote: > > > > > a. Go with phase diagram gradually changing concentrations of protein, precipitant, salt concentrations and pH. Find where you are going out of crystallisation. Define region for streak seeding. > b. Screen for ratios of precipitant to protein from 1:9 to 9:1. > > > Dr Felix Frolow > Professor of Structural Biology and Biotechnology, Department of Molecular Microbiology and Biotechnology > Tel Aviv University 69978, Israel > > Acta Crystallographica F, co-editor > > e-mail: [log in to unmask] > Tel: ++972-3640-8723 > Fax: ++972-3640-9407 > Cellular: 0547 459 608 > > > > On Feb 25, 2013, at 18:02 , lei feng <[log in to unmask]> wrote: > > > > Hello everyone, > > I need your suggestion for slowing down crystallization for my protein > > my protein got hit in PEG/ION #5 ( 0.2 M MgCl2, 20% PEG 3350, pH 5.9), but it crystallize too fast. In 1 hr I can see tons of tiny needles. > > Can anyone give me some suggestion on how to slow down the process? I used lower conc. of potein, lower conc. of PEG ( 10%), it helped a little bit, giving me small rod crystal. but no improvement after that. > > > > Thank you very much for your suggestions > > > > > > > > > > > -- Roksana OgrodowiczDivision of Molecular Structure MRC National Institute for Medical Research The Ridgeway, Mill Hill London, NW7 1AA