Hi Bhat, You can run Refmac for 0-cycles to make the maps. If you use unrestrained refinement, you don't have to mess with restraint files. There is also an option in Coot to do this. But if you are in Coot anyway, you might as well get the maps from EDS directly or (after installing a plugin: http://www.cmbi.ru.nl/pdb_redo/coot.html) get a re-refined model and map form PDB_REDO. Cheers, Robbie > -----Original Message----- > From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of > bhat > Sent: Monday, February 04, 2013 14:16 > To: [log in to unmask] > Subject: [ccp4bb] generating electron density from PDB and structure factor > file > > Dear All, > > I would like to know what is the best possible way to generate the density > from the published pdb file. > > thanks, > > Bhat