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Mark, you can try low percentage of ethanol as well, as it increases the expression of some chaperones (can't find the paper right now!). Alex > On Tue, Feb 26, 2013 at 2:08 AM, Mark J van Raaij <[log in to unmask]>wrote: > >> Dear All, >> >> We are considering trying additives to bacterial cultures to see if they >> improve solubility of some proteins we express: L-arginine, glycyl-glycine, >> sorbitol and betaine. I would be glad to receive recommendations and >> experiences, also if they haven't worked. >> Side-question: should we get cell culture-certified compounds, or would >> "normal", much more economical ones suffice? (i.e. are they likely to >> contain side-products that would affect bacterial growth?). >> >> Greetings, >> >> Mark >> >> Mark J van Raaij >> Lab 20B >> Dpto de Estructura de Macromoleculas >> Centro Nacional de Biotecnologia - CSIC >> c/Darwin 3 >> E-28049 Madrid, Spain >> tel. (+34) 91 585 4616 >> http://www.cnb.csic.es/~mjvanraaij >> >> > > > -- > David J. Leibly > Graduate Student, Yeates Lab > Dept. Chem & Biochem > Univ. of California, Los Angeles > Boyer Hall 269B > (310) 825-8901 >