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Dear Jie, my experience with the VBM by SPM using the WM is very good. I recommend you use the New Segmentation tool, which does a good job when segmenting the different tissue types, and then DARTEL for registration. For creating the template, I suggest you use two channels, one for the GM DARTEL imported files (rc1*.nii), and other for the WM DARTEL imported files (rc2*.nii). Detailed information about how performing the VBM can be found in the tutorial by prof. John Ashburner ( http://www.fil.ion.ucl.ac.uk/~john/misc/VBMclass10.pdf). Best Regards, Gemma On 29 January 2013 18:28, Jie Yu <[log in to unmask]> wrote: > Hi > I just posted a message in fsl asking about white matter VBM. > It seems like it is "never" recommended in fsl. > So I guess it is also not recommended in SPM as well? > Thanks a lot. >