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Hi Mike, check out the EZ screen builder from Emerald Bio, that is a really nice tool for this purpose: http://www.emeraldbiosystems.com/escreentoolkit/escreen.html Select your screen, your hit condition, then hit 'optimize' and you will have a screen that will vary the precipitant concentration in the horizontal and the pH in the vertical direction. You can modify the optimization screen as you like, change concentrations, the pH range, add components etc. You can then either order the screen from EmeraldBio, or download a pipetting protocol to make it from your own stock solutions. Good luck! Jan -- Jan Abendroth Emerald BioStructures Seattle / Bainbridge Island WA, USA home: Jan.Abendroth_at_gmail.com work: JAbendroth_at_embios.com http://www.emeraldbios.com On Jan 16, 2013, at 9:38 PM, Mike John <[log in to unmask]> wrote: > Hello, > > Shameful and sorry for asking this simple question, it looks like this when first starting a new setup in > so-called structural biology. > I remmeber a book of, probably, Hampton, in which there are tables of pH optimization for many buffers. For example for buffer TRIS, the table will list how many drops NaOH/HCl needed to change pH from 7.0 to 7.2, etc. This is useful for crystallization pH optimization, where can I buy or download this info? Alternatives? > > Thank you very much! > > Mike >