Does anybody have a nice, cite-able reference for the observation that GST tags can sometimes drag otherwise-aggregated/unfolding/generally unhappy proteins into solution?
We need to politely explain why a His-tagged version of our protein (purified in my lab) has activity but a GST-tagged version (purified in a different lab) doesn't.
thanks, Phoebe
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Phoebe A. Rice
Dept. of Biochemistry & Molecular Biology
The University of Chicago
773 834 1723; [log in to unmask]
http://bmb.bsd.uchicago.edu/Faculty_and_Research/