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Does anybody have a nice, cite-able reference for the observation that GST tags can sometimes drag otherwise-aggregated/unfolding/generally unhappy proteins into solution?

 

We need to politely explain why a His-tagged version of our protein (purified in my lab) has activity but a GST-tagged version (purified in a different lab) doesn't. 

 

  thanks, Phoebe

 

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Phoebe A. Rice
Dept. of Biochemistry & Molecular Biology
The University of Chicago

773 834 1723; [log in to unmask]
http://bmb.bsd.uchicago.edu/Faculty_and_Research/

http://www.rsc.org/shop/books/2008/9780854042722.asp