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-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Mike, difficult to give specific advice without knowing the crystallisation conditions. - - at the beginning in the unmixed drop your protein is soluble - - after equilibration with the mother liquor there is a crystal. Think about what is happening within the crystallisation chamber and find the spot where you don't have crystals. It must exist. I would first lower the precipitant concentration. You may instead want to lower the precipitation concentration difference by adding some of the precipitant to the protein solution (making sure the protein stays soluble). Good luck, Tim On 01/16/2013 02:30 AM, Mike John wrote: > > Hello, all, > > My crystal grows very fast even though the protein conc. is now 1.5 > mg/ml. The shape of the crystal likes a ruler plate with one side > very thin. The crystal quality has diffraction to about 3.5A in a > home source of 1.2 KW sealed tube Angilent equipment. But the data > can not be indexed due to one direction has poor diffraction and > the crystal quality. Seeking advices on improving the crystal > quality. Thank you very much > > Mike > > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFQ9puXUxlJ7aRr7hoRAhDKAJ9dW9KufS3w5pdrgKqB34LBBMKKIgCfZs5f 9gss5fNzJi+CrSAmX1kr2L0= =Z5BF -----END PGP SIGNATURE-----