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Hi, you may want to register them at first to their own MNI template (estimate --> MRI2MNI.mat and SPECT2MNI.mat), then either 1. you can overlay them in the MNI space (reslice both) or 2. concatenating the two .mat files along with inverting one of them (I am not sure how it can be done in SPM but in FSL) 2.1. MRI2MNI.mat + inv_SPECT2MNI --> MRI2SPECT to register (reslice) MRI to the SPECT or 2.2. inv_MRI2MNI.mat + SPECT2MNI --> SPECT2MRI to register (reslice) SPECT to the MRI As mentioned, it is working in FSL and should work in SPM but I am unsure how (which button(s) to press). Someone may help you out. Ave, Auer, Tibor M.D. Ph.D. MRC Cognition and Brain Sciences Unit 15 Chaucer Road Cambridge CB2 7EF United Kingdom Phone/Work: Mobile: +44-(0)7906-86-3837 Mail: [log in to unmask] -----Original Message----- From: SPM (Statistical Parametric Mapping) [mailto:[log in to unmask]] On Behalf Of Henrique Amaral Sent: Friday, December 07, 2012 12:57 AM To: [log in to unmask] Subject: [SPM] MRI - SPECT Registration Dear SPMers I have a MRI T1 image with matrix 256x256x256 (voxel size: 1x1x1) and an ictal SPECT 128x128x128 (voxel size 1.7x1.7x1.7) from the same patient with epilepsy. I´d like to register theses two images, ie, put the SPECT in the MRI space. Is it possible using the co-registration and reslice option? How could I do that? Thank you Regards. Henrique Amaral University of Sao Paulo, Brazil